Development of transplantation of hepatocytes produced from iPS cells to liver failure

开发了具有激活素A的培养基用于iPS细胞的无饲养层培养。iPS细胞从在具有制瘤素M、EGF和RA的培养基中用C/EBP α、GATA 4、HEX和FoxA2转染的iPS细胞分化成肝母细胞。肝细胞选择培养基不含葡萄糖或精氨酸，补充半乳糖和鸟氨酸。当HSM加入到iPS细胞和人原代肝细胞的共培养物中时，iPS细胞死亡，肝细胞存活。成功纯化肝细胞。

A medium with Activin A was developed for feeder-free culture of iPS cells. iPS cells differentiated to hepatoblasts from iPS cells transfected with C/EBP alpha, GATA4, HEX, and FoxA2 in a medium with Oncostatin M, EGF, and RA. Hepatocyte selection medium was developed without glucose or arginine, supplemented with galactose and ornithine. When HSM was added to co-culture of iPS cells and human primary hepatocytes, iPS cells died and hepatocytes survived. Hepatocytes were successfully purified.