99mTc-MIBI SPECT and multidrug resistance-related protein in patients with ovarian cancer

本研究的目的是评估99 mTc-MIBI SPECT对MDR相关蛋白功能成像的有用性：前瞻性研究了13名临床怀疑卵巢癌的女性。静脉注射99 mTc-MIBI 740 MBq后，分别于10 min和2 h行SPECT显像。在SPECT显像上，计算肿瘤早期显像和延迟显像的摄取率及99 mTc-MIBI的洗脱率。在手术切除的肿瘤中评估MDR蛋白的表达。免疫组化染色。6例原发性卵巢癌经组织病理学检查证实。6例卵巢癌中有5例在99 mTc-MIBI SPECT早期和延迟显像上均呈阳性。MDR相关蛋白在所有肿瘤中均有表达。99 mTc-MIBI洗脱率与YB-1表达呈显著正相关。早期肿瘤摄取率与Bax表达呈显著正相关。

The aim of this study was to evaluate the useful of 99mTc-MIBI SPECT for functional imaging of MDR-related protein: Thirteen women with a clinical suspicion of ovarian cancer were prospective studied. After intravenous injection of 740MBq 99mTc-MIBI, SPECT imaging at 10 minutes and 2 hours was performed. On the SPECT images, tumor uptake ratio of both early and delayed images, and washout rate of 99mTc-MIBI were calculated. The expression of MDR protein was assessed in surgically excised tumors. The immunohistochemical staining was performed. Six primary ovarian cancers were proven by histopathological examination. Five of the six ovarian cancers were positive on both early and delayed images with 99mTc-MIBI SPECT. The expression of MDR-related protein was found in all tumors. The washout rate of 99mTc-MIBI showed a significant positive correlation with YB-1 expression. Early tumor uptake ratio showed a significant positive correlation with Bax expression.