Non-invasive treatment of rotator cuff tear by using mesenchymal stem cells

在食蟹猴的肩部制造了4毫米和8毫米宽度的缺陷，以研究自发愈合的临界尺寸。在相邻的4mm和8mm的缺损处也建立了跨骨通道。4毫米的缺陷自行愈合，而8毫米的缺陷则没有。目前的研究表明，跨骨通道的建立逆转了8毫米缺陷的复发。分离骨髓源性间充质干细胞。在肩袖肌腱止点处形成8mm的缺损，不进行治疗（空白组）或注射MSC （MSC组）。Null组显示大部分富含软骨的修复组织覆盖缺损，这是由于过度肌腱通过软骨下骨挤压形成的。尽管产生的新修复组织较少，但MSC组表现出更好的肌腱修复质量，其表面规则并重建小梁软骨下骨。

Four-mm and 8-mm width defects were created to the shoulder of cynomolgus monkeys to investigate critical size for spontaneous healing. Transosseous passageways were also created in adjacent to the 4-mm and 8-mm defects. Four-mm defects spontaneously healed, whereas the 8-mm defects did not. The present study demonstrated that creation of the transosseous passageway reversed the recurrence of 8-mm defects.Bone marrow-derived MSCs were isolated. Eight-mm defects were created at the tendon insertion of the rotator cuff and were either left untreated (Null group) or injected with MSC (MSC group). The Null group showed most cartilage-rich reparative tissue covering the defect, owing to formation of excessive tendon extruding though the insufficient subchondral bone. Despite less amount of new reparative tissue produced, the MSC group demonstrated better quality of tendon repair with regular surface and reconstruction of trabecular subchondral bone.