The development of fertility treatment for patients with premature ovarian failure or diminished ovarian reserve by regulating activin action.

将第5.5天的小鼠新生卵巢与或不与卵清蛋白一起培养48小时。我们发现激活素β A和β B mRNA的表达减少到一半。在此基础上，我们研究了持续暴露于不同剂量的雷公藤多甙对小鼠卵巢功能的影响。每四天通过腹膜内注射将5mg溶于玉米油或玉米油中的地诺孕素给予16周龄小鼠。注射16次后，将小鼠与雄性小鼠一起圈养。比较了小鼠出生的幼崽总数。地诺孕素组明显高于对照组（P < 0.03）。 地诺孕素组末次分娩年龄也较高。我们推测地诺孕素通过抑制激活素的作用抑制原始卵泡的激活并维持原始卵泡池。这一结果将适用于包括日本在内的发达国家的卵巢储备和生育能力的维持，这些国家的妇女结婚年龄较大。现在我们来研究一下这种干扰素效应。

Mouse neonatal ovaries of day 5.5 were cultured with or without progestin for 48 hours. We found the Activin beta A and beta B mRNA expression was decreased to half. Based on the fact we researched the effect of continuous progestin exposure to mouse ovarian function. Five mg of dienogest solved in corn oil or corn oil was given by intraperitoneal injection to sixteen week old mice every four days. After sixteen times injection the mice were housed with male mice. The total numbers of pups born from a mouse were compared. It was greater in dienogest group (P < 0.03). The last delivery age was also higher in dienogest group. We speculate that dienogest suppressed primordial follicle activation and maintained the primordial follicle pool through the suppression of activin action. This result would be applicable for the maintenance of ovarian reserve and fertility in the developed country including Japan, where women get married in older age. Now we investigate this progestin effect.