Development of the method of selecting the sperm in the ART (Assisted reproductive technology)

在使用胞浆内单精子注射（ICSI）的体外受精中，将单个精子直接注射到卵子中。虽然生理分离在自然妊娠中发生，但提供给ICSI的精子是由胚胎学家选择的。我们定制了ICSI的方法和一些分析小鼠精子DNA片段的方法在我们的实验室进行，然后在本研究中检验趋化性是否可以用于哺乳动物精子分离，但是我们使用的方法无法检测趋化性。

In in vitro fertilization using intracytoplasmic sperm injection (ICSI), a single sperm is injected directly into an egg. Although the physiological separation acts in the natural pregnancy, a sperm provided to ICSI is chosen by an embryologist. We customized method of ICSI and some methods for analyses of the sperm DNA fragmentation in mice to perform in our laboratory, and then it was examined whether chemotaxis could use for mammalian sperm separation in this study, but it could not detect the chemotaxis by methods that we used.