Regeneration of pulp-dentine complex using stem cells derived from oral mucosa or peripheral blood cell in alginic acid hybrid scaffold

口腔粘膜源性细胞在含有生长因子的培养基中增殖率较低。但形成结节。在体内研究中，骨髓细胞悬浮在海藻酸凝胶中。凝胶填充于透明质酸支架的孔隙中。将支架植入同基因大鼠皮下。然而，凝胶中的成骨并没有被促进。因此，将大鼠骨髓细胞接种于热交联得到的海藻酸海绵中。将带细胞的海绵置入柱状多孔透明质酸支架中空中心，植入大鼠皮下观察成骨效果。结果，骨钙素含量显著升高。成骨量明显增加。在透明质酸与福尔马林处理的聚乙烯醇海绵复合支架中，涂覆层粘连蛋白、亮氨酸、色氨酸或溶酶，体内检查发现骨髓细胞成骨量明显增加。

Oral mucosa-derived cells showed low rate of proliferation in the culture containing growth factors in the medium. However, nodule was formed.In vivo study, bone marrow cells were suspended in alginic acid gel. The gel was filled in pores of HA scaffolds. The scaffolds were implanted in the subcutis of syngeneic rats. However, osteogenesis in the gel was not promoted. Therefore rat bone marrow cells were seeded in alginic acid sponge obtained by heat crosslinking. The sponge with the cells was inserted in the hollow center of cylindrical porous HA scaffold and implanted in rat subcutis for assessment of osteogenesis. As the result, quantity of osteocalcin significantly increased. Moreover, osteogenesis significantly increased quantitatively.In hybrid scaffold composed of HA and formalin treated polyvinyl alcohol sponge coated with laminin, leucine, tryptophan or lysin, the in vivo examination revealed the significant quantitative increase of bone formation by bone marrow cells.