Therapeutic Interaction of Systemically-administered Mesenchymal Stem Cells with Peri-implant Mucosa

间充质干细胞(MSCs)具有多向分化能力和调节功能，尤其是在炎症部位，是再生医学中极具吸引力的细胞来源。在这里，我们研究了系统间充质干细胞移植对种植体周围上皮封闭的影响。将MSCs经尾静脉系统注射到受体大鼠体内。移植的MSCs在损伤的牙龈黏膜处聚集，随后口腔上皮关闭，种植体周上皮(PIE)早于对照组形成。此外，在MSC-种植体组，在第4周时，在PIE-种植体的界面上检测到层粘连蛋白332(Ln)阳性层。相反，对照组仅在根尖区和结缔组织区可见Ln。系统间充质干细胞移植可能有效地增强钛种植体周围的上皮封闭性。

Mesenchymal stem cells (MSCs) are an attractive cell source in regenerative medicine because of their multi-differentiation capacity and regulatory functions, specifically at sites of inflammation. Here, we investigated the effect of systemic MSC transplantation on peri-implant epithelial sealing. MSCs were systemically injected into recipient rats via the tail vein. The injected MSCs accumulated at the injured gingival mucosa, following which the oral epithelium closed and peri-implant epithelium (PIE) formed from the oral sulcular epithelium earlier than in control rats. Furthermore, in the MSC-implant group, a laminin 332 (Ln)-positive layer was detected along the interface of the PIE-implant at 4 weeks. Conversely, Ln was only observed in the apical region and connective tissue region in the control group. Systemic MSC transplantation may be effective for enhancing epithelial sealing around titanium implants.