Effects of food ingredients to cariogenic biofilms and alpha-glucans.

葡聚糖被认为是口腔生物膜形成的锚定分子。我们用葡聚糖固定培养板进行体外培养，证实了这一假设。独立制备α -1,3-葡聚糖、α -1,6-葡聚糖和α - 1,3,1,6 -葡聚糖并固定在96孔板的孔上。用无蔗糖培养基在该平板上培养变形链球菌，0 /N孵育后观察生物膜的形成。有趣的是，变形链球菌在葡聚糖板上的初始附着比在唾液涂层板上的附着弱。其他口腔链球菌也进行了检测，但未观察到像变形链球菌那样形成生物膜。通过表达GTFs的大肠杆菌转化子，研究了GTFs对生物膜形成的作用。这些大肠杆菌可以在葡聚糖固定板上形成生物膜。在研究过程中，我们发现可溶性α -葡聚糖葡聚糖可以作为生物膜脱植底物。

The alpha-glucans are thought to be an anchor molecule for formation of oral biofilms. We confirmed this hypothesis by in vitro culture using the glucan fixed culture plate. The alpha-1,3-glucan, alpha-1,6-glucan and alpha-1,3-1,6-glucan are independently prepared and fixed on the wells of 96-well plates. Streptococcus mutans was cultured on this plate by the sucrose free medium, and the biofilm formation was observed after O/N incubation. Interestingly, initial attachment of S. mutans on the glucan plate was weaker than on the saliva coating plate. The other oral Streptococci were also tested but biofilm formation were not observed as like as S. mutans. The function of GTFs to biofilm formation were investigated by E. coli transformants expressing GTFs. Those E. coli could form biofilms on the glucan fixed palte.During the research, we found that soluble alpha-glucan, dextran, could be used as biofilm decolonizing substrate.