Comparative chromosome painting in Brassicaceae for identification of chromosome homeology and elucidation of karyotype evolution

利用染色体特异的BAC重叠群FISH技术对拟南芥的整个染色体进行染色和比较染色体染色(CCP)已成功地用于鉴定相关十字花科植物减数分裂阶段的同源染色体(粗线期)。在这些先决条件的基础上，它打算将CCP应用于芥科8个部落的~50个分类群，包括拟南芥属的近亲。其目的是揭示与拟南芥属5条染色体同源的染色体/染色体区域，以及在进化过程中发生的、导致所选物种基本染色体数目(X=4至10)变化的染色体重排。这是CCP在植物家族中的第一次应用，将通过染色体计数(新的或确认的)、通过流式细胞仪估计基因组大小以及通过FISH鉴定携带染色体的rDNA来补充。染色体同源性的数据有望与最近的分子系统发育数据相比较，阐明十字花科植物的核型进化和系统发育关系。

Painting of entire Arabidopsis thaliana chromosomes by FISH with chromosomespecific BAC contigs and comparative chromosome painting (CCP) for identification of homeologous chromosomes in meiotic stages (pachytene) of related Brassicaceae species have been established successfully. Based on these prerequisites it is intended to apply CCP to ~50 taxa of eight tribes of the mustard family including the closest relatives of the Arabidopsis genus. The aim is to reveal chromosomes/chromosome regions homeologous to the five A. thaliana chromosomes and chromosome rearrangements that occurred during evolution and contributed to the variation of the basic chromosome numbers (X=4 to 10) among the selected species. This first application of CCP to a plant family will be supplemented by chromosome counts (new or confirmative), genome size estimation by flow-cytometry, and identification of rDNA bearing chromosomes by FISH. The data on chromosome homeology are expected to elucidate karyotype evolution and phylogenetic relationships within the Brassicaceae in comparison with recent molecular phylogenetic data.