Development of screening method to isolate molecules that regulate behaviors of neurotransmitter receptors at synaptic membranes

十二烷基硫酸钠消化的冷冻骨折复制品标记电子显微镜(SDS-FRL EM)是一种能够在保持空间结构的情况下显示突触膜上的蛋白质，如神经递质受体的技术。然而，由于对转基因神经元的识别困难，这种结合基因转染的技术还没有建立起来。在这个项目中，我们开发了一种新的技术来区分复制膜上的转基因神经元，方法是在十二烷基硫酸钠消化后引入膜系留荧光蛋白作为标记。

SDS-digested freeze-fracture replica labeling electronmiroscopy (SDS-FRL EM) is a technique that enables visualization of proteins, such as neurotransmitter receptors, on synapse membrane with maintenance of space structure. However, the technique in combination with gene transfection has not been established because of the difficulty in recognition of transfected neurons. In this project, we developed a new technique to distinguish transfected neurons on replica membranes by refolding membrane tethered fluorescent proteins that are introduced as markers after SDS digestion.