Development of high functional hydrogel as biopaper for 3D bioprinter and application for tissue engineering

在这项研究中，我们开发了用于3D生物打印机的高功能水凝胶材料作为生物墨水和生物纸。通过喷射含有细胞的预凝胶溶液，可以制造3D组织样结构。我们使用藻酸盐溶液作为胶凝材料，然而众所周知，藻酸盐不适合用作细胞粘附支架。因此，我们分别用胆固醇对海藻酸钠进行改性以增加疏水性，用环糊精对明胶或其他生长因子进行改性以降低胆固醇。因此，我们成功地制备了藻酸盐凝胶，其中环糊精修饰的材料可以吸附到表面上。此外，观察到可以在具有明胶斑点区域的藻酸盐凝胶上制备细胞图案化表面。

In this research, we developed high functional hydrogel materials as bioink and biopaper for 3D bioprinter. It is possible to fabricate 3D tissue-like structures by ejecting pre-gel solution with cells. We used alginate solution as gelling materials, however it is well-known that alginate is not suitable for using as a cell-adhesive scaffold. Therefore, we modified alginate with cholesterol for adding hydrophobicity, and gelatin or other growth factors with cyclodextrin which can subsume cholesterol, respectively. And thus, we succeeded to fabricate alginate gel where cyclodextrin-modified materials can adsorb onto the surface. Moreover, it was observed that cell-patterning surface could be prepared onto the alginate gel with gelatin spotted areas.