Structural and functional studies on Chloroethene sensor kinase and its application for bioremediation

氯乙烯是土壤和地下水中最严重的污染物之一，其检测和去除系统的建设是一个亟待解决的重要问题。本研究以氯乙烯脱氯为模板，根据已知的氯乙烯敏感蛋白的氨基酸序列设计了一组引物，通过聚合酶链式反应扩增了氯乙烯敏感蛋白的基因。将获得的传感器蛋白在大肠杆菌中表达，并对其结构和功能进行了研究。

Chloroethene is one of the most serious pollutants of soil and underground water, and the construction of its detection and removal systems is a very important problem to be solved. In this study, genes of chloroethene sensor kinase were amplified by PCR with a primers set designed based on amino acid sequences of the known chloroethene sensor kinase using a metagenome prepared from a consortium dechlorinating chloroethene to ethene as a template. The obtained sensor kinase was expressed inEscherichia coli and their structures and function were studied.