Neuronal uptake and control of neurite outgrowth by carbon nanotubes

与可溶性NGF一样，CNTs包被NGF促进神经元突起的生长。ngf包被的碳纳米管和氨基修饰的碳纳米管都被纳入神经元，并在ERK信号通路中促进磷酸化的ERK。CNTs通过PLC信号通路中的PKC磷酸化ERK，而不依赖于ERK信号通路中的Ras/Raf/MEK级联。接下来，我们研究了将ngf包被的碳纳米管附着在金气相沉积基底上对神经元神经元网络形成的调控。在宽度为50 ~ 100 μm的金气相沉积区域上，神经元粘附、伸长并形成神经元网络。

CNTs coated with NGF promoted the neurite outgrowth of neurons in the same manner as soluble NGF. Both NGF-coated CNTs and amino group-modified CNTs were incorporated into the neurons and promoted phosphorylated ERK in the ERK signaling pathway. CNTs phosphorylated ERK through PKC in the PLC signaling pathway independent of the Ras/Raf/MEK cascade involved in the ERK signaling pathway. Next, we investigated the regulation of neuronal network formation of neurons using NGF-coated CNTs attached to gold vapor deposition substrates. Neurons adhered, elongated neurites and formed neuronal networks on the gold vapor deposition regions with 50-100 μm in width.