Identification of cell adhesion molecules and growth factors in the serum & the development of new culture medium

通过本研究，我们对包括FBS在内的多种动物血清中的血细胞粘附性和抑制生长因子进行了鉴定，并对其增殖潜力价值进行了评估，得到了以下结果：多种哺乳动物的失活血清对浮血细胞系统细胞具有粘附抑制活性，结果表明主要的粘附抑制因子是补体C4bp、H因子和脂蛋白LDL/VLDL。C4bp、H因子和LDL/VLDL优先覆盖培养瓶实验室培养皿疏水表面的血清蛋白，并推测是什么检查了浮动血细胞细胞可能不会粘贴非特异性。对血清中的血细胞生长抑制因子进行了鉴定，其中一种是IgM，发现对氧化LDL也有很强的细胞毒性，使LDL升温（血清失活条件56℃，30分钟），产生细胞毒性。另一方面，未发现对IgG有明显抑制作用。Jurkat在T淋巴细胞存量中LDL需求性强，而Molt-4的LDL需求性低，产生胰岛素、转铁蛋白，仅增加亚硒酸（ITS）。此外，Jurkat的完美乘法发现除了生长因子或脂质之外的低分子成分也是所需的东西。如果去除IgM以使Jurkat对一个指数进行增殖，并且LDL的数量可以控制，则有可能获得与FBS相等的替代培养基。

By this research, we performed identification of the blood cell cell adherence and the inhibitory growth factors in various animal sera including FBS, and evaluation of proliferation potential value, and obtained the following results.1. The inactivation serum of various mammals has adhesion inhibitory activity over a floating blood cell system cell, and it turned out that the main a dhesion prevention factors are complement C4bp, H factor, and lipoprotein LDL/VLDL. C4bp, and H factor and LDL/VLDL covered the hydrophobic surface of the cultivation flask laboratory dish preferentially in serum protein, and what is checked so that a floating blood cell cell may not paste up nonspecific was conjectured.2. Identification of the blood cell cell-growth inhibition factor in serum was advanced, and one of them is IgM and it found out cytotoxicity strong also against the oxidization LDL which heats LDL (56 ℃ of inactivation conditions of serum, 30 minutes), and arises. On the other hand, inhibitory activity remarkable in IgG was not able to be found out.3. LDL demand nature of Jurkat was strong in the T -lymph cell stock, and on the other hand, Molt-4 had low LDL demand nature and it turned out an insulin, transferrin, and that only selenious acid (ITS) is increased. Moreover, perfect multiplication of Jurkat found low molecular components other than a growth factor or lipid also for the required thing. If IgM is removed for multiplication of Jurkat against an index and the amount of LDL(s) can be controlled, it is possible that development of the alternative culture medium which is equal to FBS is attained.