Creation of minimal artificial virus replication system by totally chemical syntheses

本研究的目的是构建由合成短DNA和肽纳米胶囊（合成病毒衣壳）组成的最小人工病毒。主要研究结果如下：1)成功构建了将M13噬菌体DNA包封在β环肽纳米胶囊内的人工病毒。2)设计了编码-环空肽的DNA，但通过无细胞蛋白合成系统无法获得目标肽。3)通过dna -环空肽偶联物的自组装，构建了dna修饰的人工病毒衣壳。

The aim of this work is construction of minimal artificial virus that consists of synthetic short DNA and peptide nanocapsule (synthetic viral capsid). The important results are as follows: 1) We succeeded in construction of artificial virus that encapsulated M13 phage DNA in the beta-annulus peptide nanocapsule. 2) DNA that encoded beta-annulus peptide was designed, but the objective peptide could not be obtained by cell-free protein synthesis system. 3) DNA-modified artificial viral capsid was able to be constructed by self-assembly of DNA-beta-annulus peptide conjugate.