Development and application of "artificial biotin" derivatives using methodologies of chemical biology

为了进一步完善作为化学生物学中通用结合对的生物素-链霉亲和素系统，我们筛选了大约160,000个化合物的大型文库，为链霉亲和素的新配体。因此，我们成功地获得了与蛋白质具有1μM量级亲和力的新配体，并通过X射线结晶学和计算机模拟详细分析了它们的结合机理。

In order to further sophisticate biotin-streptavidin system that is used as a universal binding pair in chemical biology, we screened a large library of approximately 160,000 compounds for novel ligands to streptavidin. As a result, we succeeded in obtaining new ligands that have affinities to the protein in the order of 1 μM. Moreover, we analyzed their binding mechanisms in detail by X-ray crystallography and computer simulation.