Elucidation of mechanism of the hyperuricemia accompanied by pregnancy induced hypertension (PIH) and multiple pregnancy.

在这项研究中，我们比较了母体血清尿酸水平和脐带血尿酸水平，并研究了滋养层细胞系BeWo细胞的尿酸转运机制。母血和脐血之间的血尿酸水平没有显著差异，这表明尿酸在胎盘中可以自由移动。RT-PCR和免疫组织化学结果显示，尿酸转运蛋白OAT4、OAT10、URATv1和ABCG2在胎盘合体滋养层细胞和BeWo细胞中均有表达。尽管表达尿酸转运体，BeWo细胞并不摄取尿酸。在确认这些细胞在跨井培养上形成紧密连接后，测量上下室之间的尿酸盐转运。尿酸盐在BeWo细胞单层中以非饱和动力学运动，即使在4℃孵育时也能观察到这种运动，这表明尿酸盐通过简单的扩散途径通过细胞旁路。

In this study, we compared maternal serum urate levels with those of umbilical cord blood and investigated urate transport mechanisms in BeWo cells, a trophoblast-derived cell line. There were no significant differences in serum urate levels between maternal blood and umbilical cord blood, suggesting that urate is freely movable at the placenta. RT-PCR and immunohistochemistry showed that urate transporters including OAT4, OAT10, URATv1 and ABCG2 were expressed in the syncytiotrophoblast cells in the placenta as well as BeWo cells. Despite expressing urate transporters, BeWo cells did not take up urate. After confirming the formation of tight junctions of these cells cultured on the transwell, urate transport between upper and lower chambers was measured. Urate moved through BeWo cell monolayers with non-saturation kinetics and this movement was observed even when the cells were incubated at 4 degree, suggesting that urate moves through the paracellular route by simple diffusion.