The pathologic clarification of cervical cancer based on epigenetic regulation

我们研究了L1基因甲基化状态与HPV52/58和CIN长控制区(LCR)的相关性。HPV52/58L1基因CpG位点甲基化增加与宫颈病变的严重程度相关。提示L1基因甲基化状态是CIN的候选标志物，可用于CIN2和CIN3的检测。阐明CIN病变中的抗肿瘤免疫状态对于阐明宫颈癌的致癌机制具有重要意义。我们调查了细胞诊断结果和HPV感染之间的关系。CIN患者外周血中GM-CSF的表达随病变进展而增加，提示CIN可诱导宿主抗肿瘤免疫反应。另一方面，CIN组织中MCP-1的表达随病变进展而减少，提示CIN组织中存在免疫逃逸现象，可能与CIN的进展有关。

We investigated the correlation between methylation status in the L1 gene and the long control region (LCR) of HPV52/58 and CIN. The increased methylation at the CpG sites in the HPV52/58 L1 gene was correlated with the severity of cervical neoplasia. These data suggest that HPV methylation status of the L1 gene is a candidate biomarker of CIN for detecting CIN2 and CIN3.The elucidation of antitumor immunity in CIN lesion is important for the elucidation of carcinogenic mechanism in cervical cancer. We investigated associations with the results of cytodiagnosis and HPV infection. It appeared that GM-CSF expression increased with lesion progression in the CIN cases, and the results suggested that host antitumor immune response was induced. On the other hand, MCP-1 expression decreased with lesion progression in the CIN cases, and the results suggested that immune escape developed, and that may be correlation with the progression in the CIN.