Application of urokinase for inner ear therapy

尿激酶是临床上用作溶栓剂的药物。近年来，有研究报道了尿激酶的抗凋亡作用。在本研究中，我们研究了尿激酶是否对内耳细胞具有抗凋亡作用。我们用耳蜗器官型培养证明尿激酶能减轻氨基糖苷引起的内耳毛细胞死亡。在尿激酶与氨基葡糖苷同时给药组可见P38的表达，提示尿激酶通过激活P38 MAPK通路发挥抗凋亡作用。我们还发现尿激酶降低了调节肽引起的螺旋神经节细胞死亡。这些结果表明，尿激酶可能是一种有前途的内耳治疗药物之一。

Urokinase is a drug used clinically as a thrombolytic agent. Recently, the anti-apoptotic effect of the urokinase has been reported. In this study, we have examined weather urokinase has the anti-apoptotic effect to the inner ear cells. We have shown that urokinase attenuated the inner ear hair cell death caused by aminoglicoside using the cochlear organotypic cultures. Expression of P38 could be seen in those who had given urokinase concomitantly with aminoglicoside, which suggested that urokinase showed antiapoptotic effect by activating the p38 MAPK pathway. We also showed that urokinase decreased the spiral ganglion cells death caused by gulutate. These result showed that urokinase could be one of a prommising drug for the inner ear therapy in future.