"What is the keloid diathesis? "-Role of Fibrocytes in Keloids -

瘢痕疙瘩通常很难治疗。瘢痕疙瘩形成的机制尚不完全清楚。由于多发性和复发性病变经常发生在瘢痕疙瘩患者中，我们假设全身异常与其发病有关。我们分析了瘢痕疙瘩患者外周血中的成纤维细胞前体细胞，称为“循环纤维细胞(CFs)”。瘢痕疙瘩患者CFs特征的改变可能在瘢痕疙瘩的发病机制中起重要作用。本研究的目的是采集瘢痕疙瘩患者和健康志愿者的血样。为了从外周血中分离CFs，用Ficoll Hypaque密度梯度离心法从全血(10ml~20ml)中分离外周血单个核细胞(PBMC)，并在DMEM中进行培养。另一方面，对瘢痕疙瘩患者的组织进行培养，分离成纤维细胞。在此之前，我们利用NOD SCID小鼠对创面愈合与成纤维细胞的关系进行了体内实验。对NOD SCID小鼠背部进行损伤，造成φ5 mm皮肤缺损，尾静脉注射人CFs。取小鼠背部瘢痕组织进行免疫组织化学染色。7d后，CFS对瘢痕组织中的人HLA-a、b、c抗体进行染色。与正常皮肤相比，伤后14d人GAPDHmRNA、人转化生长因子-β-1mRNA表达增强，CFs在创面迁移，多种细胞因子表达。以及对伤口愈合的影响。我们将能够控制异常的CFS，它可能有助于瘢痕形成的治疗。

Keloids are often difficult to treat . The mechanisms underlying keloid formation are only partially understood. Because multiple and recurrent lesions frequently occur in patients with keloids, we hypothesize that systemic abnormalities are involved in the pathogensis. We analyzed fibroblast progenitor cells, called “circulating fibrocytes(CFs)”, in the peripheral blood of keloid patients. The altered characteristics of CFs from patients with keloids may have an important role in the pathogenesis of keloids. The aim of this study, Blood samples were obtained from the pat ients with keloids, and the healthy volunteers. To isolate CFs from peripheral blood, peripheral blood mononuclear cells (PBMCs) were separated from whole blood (10ml ~20ml) by Ficoll Hypaque density -gradient centrifugation, and cultured in DMEM. On the other hand, the tissue from keloids patients were cultured with previous methods and isolated fibroblasts. Before that time , we made experiment in vivo for the relation between the wound healing and fibrocytes using NOD scid mice. We injured for the back of NOD scid mice, making the φ5mm skin defects, and then injected the human CFs from the tail vein. Imunohis tochemistry was performed from the back scar tissue of the mice. CFs stained human HLA -a,b,c antibody from the scar tissue after 7days. The mRNA expression of humanGAPDH, humanTGF -β1 were increased after 14days, compared with normal skin.CFs were migrate in the wound site with expression of the various cytokines. And influence on wound healing. We will able to control of the abnormal CFs, its might be useful for the treatment for scar formation.