Basic study of soft tissue augmentation by adipose-inductive biomaterial

重建手术包括自体组织移植的体积扩大。然而，作为供体部位的健康区域被破坏，自体组织像拼凑一样被转移。我们试图在人工生物材料中诱导成脂活性，这种材料可以通过注射针进行组织增强注射。首先，以脂肪前体细胞为研究对象，考察试剂对PPAR-g基因表达水平的影响，确定最佳剂量的盐酸吡格列酮。然后，将含有吡格列酮的鲑鱼胶原蛋白按剂量进行调整，并用注射针将鲑鱼胶原蛋白注射到小鼠背部。植入后4周，用成熟脂肪细胞替代吡格列酮胶原凝胶，与未植入吡格列酮的对照胶原凝胶比较。这些结果表明使用胶原蛋白和吡格列酮作为脂肪诱导物质促进脂肪生成的可能性。

Reconstructive surgery involves volume augmentation with autologous tissue transfer. However, a healthy region is damaged as a donor site, and the autologous tissue is transferred like a patchwork. We have attempted to induce adipogenesis activity in artificial biomaterial that is injectable with an injection needle for tissue augmentation. First of all, the optimal dose of pioglitazone hydrochloride was examined with adipo-precursor cells in terms of the PPAR-g mRNA expression levels affected by reagent in vitro. Then, salmon collagen with pioglitazone was adjusted in terms of the dose and the salmon collagen was injected into mouse back using an injection needle in vivo. At 4 weeks after implantation, the pioglitazone collagen gel was substituted by mature adipocytes in comparison with the case for control collagen gel without pioglitazone. These results are indicative of the possibility of promoting adipogenesis using collagen with pioglitazone as an adipose-inductive substance.