An Experimental Study on Development of Cultured Epithelial Grafting Sheets with Amnion and Oral Epithelial Cells

本研究的目的是评估人口腔粘膜上皮细胞在超干羊膜（培养片）上可用作培养复层上皮片的新来源的可能性。培养片有一个分层良好的上皮层类似于未培养的粘膜。免疫组化显示，培养片由高度活跃的增殖细胞和未分化细胞组成，具有屏障功能。这些结果表明，培养的复层上皮片可能是一个有前途的发展复层上皮片。这些培养的片材不仅可用于牙科治疗，而且可用于包括治疗烧伤等在内的广泛领域。

The aim of this study was to assess the possibility of human oral mucosal epithelial cells on hyper-dry-amnion (cultured sheets) can be used as new source for culturing stratified epithelium sheets. Cultured sheets had a well-stratified epithelial layer similar to that of uncultured mucosa. Immunohistochemistry, cultured sheets consisted of highly-active proliferating cells and undifferentiated cells, and had a barrier function. These results suggested that the cultured sheets may be a promising for the development of stratified epithelium sheets. These cultured sheets may be clinically applicable in not only dental treatment but also broad fields including the treatment of burns etc.