Study on establishment of the molecular biologic basewhich assumed mTOR for the periodontal regeneration.

在牙周再生治疗中，我们认为重要的是控制牙周组织中的宿主细胞，并将细胞移植到适合再生的状态。在本研究中，我们试图利用哺乳动物靶点雷帕霉素(MTOR)来建立控制细胞达到适合牙周再生的状态的分子生物学基础。因此，我们观察到FK506通过FKBP12的Smad信号通路促进BMP-2诱导的成骨细胞分化。此外，在人牙龈成纤维细胞(HGFiPS)诱导的多能干细胞培养体系中，我们观察到一些mTOR抑制剂的加入改变了细胞的定植和基因表达模式。这一结果可能表明mTOR通路在hGFiPS中起着关键作用。

In the periodontal regenerative therapy, we have considered that it is important to control host cells in periodontium and transplant cells to a state suitable for regeneration. In this study, it was intended that we established the molecular biologic base that controlled a cell to a state suitable for the periodontal regeneration by using the mammalian target of rapamycin (mTOR). Asa result, we had observed that FK506 enhanced BMP-2-induced-osteoblastic differentiation through smad-signaling pathway via FKBP12 on human cementoblasts (HCEM). Furthermore, in the induced pluripotent stem cells derived from a human gingival fibroblast (hGFiPS) culture system, we had observed that the colonization and gene expression patterns were altered in the addition of some mTOR inhibitors. This result may indicate that mTOR pathway plays a pivotal role in the hGFiPS.