Development of High Performance Enzyme Activity Assay for Glycoside Hydrolase Using Metal-ions as Indicator

本研究以铜酞菁络合物修饰纤维素，建立了一种简便的纤维素酶测定方法，并采用电感耦合等离子体发射光谱法（ICP-OES）或无焰原子吸收光谱法（FL-AAS）进行了替代。 然后，尽管纤维素酶在分子中不包括金属离子，但金属离子通过铜（II）-酞菁络合物改性的纤维素的生物消化而释放到溶液中。 与普通的分光光度法相比，该方法能在短时间内（15 min内）测定纤维素酶的浓度和酶活。

In this study, the simple determination method of cellulase has been developed using copper(II)-phthalocyanine complex modified cellulose with the alternative of an inductively coupled plasma -optical emission spectrometry (ICP-OES) or flame-less atomic absorbance spectrometer(FL-AAS). Then, although the cellulase does not include the metal ion in the molecule, the metal ion is released by the biodigestion of copper(II)-phthalocyanine complex modified cellulose into the solution. The presented method can measure the cellulase concentration and the activity in short time (within 15min) as comparing with common spectrophotometric method.