Development ofa novel method for analyzing various DNA methylation

微生物含有各种甲基化的DNA物种，如5-甲基胞嘧啶、n6 -甲基腺嘌呤和n4 -甲基胞嘧啶，它们是由DNA限制性修饰系统产生的。为了开发一种广泛分析DNA甲基化位点的新方法，我设计了从I型限制性修饰系统（免疫沉淀/PCR，甲基化质粒库/HPLC，甲基化质粒库/western blot）中产生的n6 -甲基ladenine位点的免疫学方法并对其进行了评估。详细分析表明，“甲基化质粒文库/western blot”方法对ⅰ型甲基化位点分析是有效的。

Microbes harbor various methylated DNA species, such as 5-methylcytosine, N6-methyladenine, and N4-methylcytosine, which are produced by DNA restriction-modification systems. Aiming at the development of a novel method for widely analyzing DNA methylation sites, I designed immunological methods to analyze N6-methyladenine sites arising from type I restriction-modification systems (immunoprecipitation/PCR, methylated plasmid library/HPLC, methylated plasmid library/western blot) and evaluated them. Detailed analysis suggested that the method “methylated plasmid library/western blot” was effective for analyzing type I methylation sites.