Molecular mechanisms of primary ciliary resorption and cilia-dependent cell cycle regulation.

初生纤毛是许多细胞类型的G0期出现的非运动性感觉细胞器，作为细胞触角接受各种环境刺激。纤毛功能的破坏会导致许多人类疾病和遗传性疾病，统称为纤毛疾病。虽然初级纤毛对感觉是必不可少的，但人们对控制纤毛吸收的细胞机制知之甚少。我之前的研究表明，胞浆动力蛋白复合体的轻链之一Tctex-1的磷酸化在导致原发纤毛吸收中发挥了重要作用。在本研究中，我进一步认为Tctex-1通过肌动蛋白聚合和内小体形成来调节原发纤毛吸收和细胞周期重入。胞膜蛋白是一种与内体形成有关的分子，分布于睫毛区。

Primary cilia are nonmotile sensory organelles presented during the G0 phase of many cell types, and act as cellular antennae to receive a variety of environmental stimuli. Disruption of cilia function results in numerous human diseases and genetic disorders collectively called 'ciliopathies'. Although primary cilia are essential for sensing, little is known about the cellular machinery controlling the resorption of cilia. I previously showed that phosphorylation of Tctex-1, one of a light chain of cytoplasmic dynein complex, played an important role in resulting primary ciliary resorption. In the present study, I further suggested that Tctex-1 regulated primary ciliary resorption and cell cycle reentry through actin polymerization and endosome formation. Clathrin, a molecule involved in endosome formation, was distributed on periciliary region.