Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisome

许多膜蛋白被募集到细胞分裂部位，并驱动大肠杆菌的细胞分裂。膜蛋白相互作用，形成巨大的蛋白质复合体，称为分裂组。目前，该复合体在细胞膜上的结构和分裂体形成的基本机制尚不清楚。在本研究中，我们开发了一种新的模型膜，可以分析一个巨大的膜蛋白复合体的结构和功能，并试图揭示大肠杆菌细胞分裂的分子机制。利用大肠杆菌提取的脂类，在液-气界面成功地制备了扁平脂膜。我们制备了一些驱动细胞分裂的GFP融合膜蛋白，并将其重组成模型膜。在本研究结束时，我们没有发现膜蛋白进入模型膜。最近，我们发展了一种从培养细胞中制备细胞膜片的新方法。我们将继续尝试使用模型膜来分析膜蛋白复合体。

Many of membrane proteins are recruited at cell division site and drive the E. coli cell division. The membrane proteins interact with each other and forms huge protein complex named divisome. At present, fundamental mechanism of divisome formation and structure of the complex on cell membrane has not been clear. In this study, we developed a new model membrane, which can analyze a structure and functions of huge membrane proteins complex, and tried to reveal molecular mechanism of E. coli cell division. We succeeded in flat lipid membrane at liquid-air interface using E. coli extracted lipid. We prepared some GFP fusion membrane proteins, which drive cell division, and reconstituted into the model membrane. At the end of this study period, we could not find membrane protein into the model membrane. Recently, we developed a new method for preparation of cell membrane sheet derived from cultured cell. We will keep trying to analyze membrane protein complex by using the model membrane.