Cloning of wheat vernalization gene Vrn-D4 and its functional analysis

为了剖析小麦的春化机制，需要对所有春化基因进行分子克隆和功能分析。在本研究中，我们重点研究了春化基因VRN-D4。我们构建了VRN-D4基因座周围的高密度遗传图谱，将VRN-D4区域缩小到0.09 cM区间。通过参考Brachypodium相应区域的基因组信息，我们选择了VRN-D4候选基因。然而，没有发现显著的变化。

To dissect the wheat vernalization mechanism, molecular cloning of all vernalization genes and their functional analyses need to be conducted. In this study, we focused on the vernalization gene Vrn-D4. We constructed the high density genetic map around the Vrn-D4 locus, which narrowed down the Vrn-D4 region to the 0.09cM interval. By referring the genomic information on the Brachypodium corresponding region, we selected Vrn-D4 candidate genes. However, no significant variation was found.