Study of relationship between antioxidant activity of soybean flavonoids and chilling stress, and their related gene

我们和其他人的研究表明，控制短柔毛颜色的T位点对应的大豆F3'H基因与大豆的低温胁迫有关。本研究旨在通过形态研究和黄酮类化合物分析，阐明转化大豆F3'H与低温胁迫的关系及其机制。通过RT-PCR和HPLC分析证实了该基因的表达。形态学研究和DPPH自由基的清除活性表明，F3'H定位于未成熟种皮的种脐和种皮的张力质体，影响这些组织中类黄酮的生物合成和抗氧化性能。

Our studies and others have suggested that soybean F3`H gene corresponding to the T locus, which controls pubescence color, is related to chilling stress in soybean. The aim of this study was to clarify the relationship between F3`H and chilling stress using transformed soybean, and its mechanism by morphological study and analyzing flavonoids. A transformant was obtained and gene expression was confirmed by RT-PCR and HPLC analyses of flavonoids. Morphological study and scavenging activity of the DPPH radical indicated that F3`H is localized to the tonoplast in the hilum and funiculus of the immature seed coat, affecting biosynthesis of flavonoid and antioxidant properties in those tissues.