Structural and functional analyses of capsid protein and RNA polymerase in the candidate virus for honeybee colony collapse disorder

蜂群衰竭失调症（Colony Collapse Disorder，CCD）是一个严重威胁蜜蜂健康和商业养蜂及授粉业经济稳定的问题。在这项研究中，我们专注于以色列急性麻痹病毒（IAPV），这被认为是一个候选的原因CCD，并提出了挑战，以调查的结构和功能的衣壳蛋白和RNA聚合酶在IAPV。通过2种或3种亚基蛋白的组合表达，表达、溶解和纯化衣壳蛋白的重组蛋白。接下来，我们从蜜蜂的脑和内脏中发现了2种蛋白质，它们似乎与这些重组衣壳蛋白亚基相互作用。6种RNA聚合酶部分重组蛋白中有2种成功表达并溶解。我们将尝试分离蜜蜂组织中与这些重组蛋白相互作用的蛋白质。

Colony Collapse Disorder (CCD) is a serious problem threatening the health of honeybees and the economic stability of commercial beekeeping and pollination operations in the world. In this study, we focused on Israeli Acute Paralysis Virus (IAPV), which is considered as a candidate reason for CCD, and challenged to investigate the structure and function of the capsid protein and the RNA polymerase in IAPV. Recombinant proteins of the capsid proteins were expressed, solubilized and purified by combinational expression of 2 or 3 kinds of subunit proteins. Next, we found 2 proteins, which seemed to interact with these recombinant capsid protein subunits, from honeybee brains and guts. Two of 6 partial recombinant proteins of RNA polymerase was successful for expression and solubilization. We will try to isolate the proteins in honeybee tissues which interact with these recombinant proteins.