Identification and functional analysis of O-glycosylated proteins in filamentous fungi

o糖基化蛋白在真菌的细胞形态发生中起着重要的作用。我们对推测的细胞壁完整性（CWI）信号的应激传感器蛋白——mid2样蛋白（MidB）进行了表征。MidB的破坏表明其对刚果红和钙萤光等细胞壁抑制剂的敏感性增加，表明MidB参与了麻豆的CWI。然而，MidB对于下游MpkA-RlmA信号传导并不是必需的，而MpkA-RlmA信号传导会导致agsBgene的瞬时转录上调。

O-glycosylated proteins play an important role in the cell morphogenesis of fungal species. We characterized the putative stress sensor protein ofcell wall integrity (CWI) signaling, Mid2-like protein (MidB), in the Aspergillus nidulans. The disruption of midBshowed the increased sensitivities to the cell wall inhibitors such as Congo red and calcofluor, indicating that the MidB is involved in the CWI in the A. nidulans. However, the MidB was not essential for the downstream MpkA-RlmA signaling that caused a transient transcriptional up-regulation of the agsBgene.