Investigation of apoC-III on atherosclerosis

家兔的脂蛋白代谢特点与小鼠不同，与人相似，我们拟制备人apoCIII转基因（Tg）家兔，以揭示其对脂蛋白代谢和动脉粥样硬化的作用。第一年，我们注射了500多个胚胎，但由于出生的幼崽数量少，没有获得任何阳性的兔子。之后，我们修改了显微注射方案。第二年，我们成功地得到了48只活着的幼崽。虽然我们还没有得到任何携带人类apoCIII基因的阳性兔子，但我们很有可能很快就会成功。进一步实验的工作正在进行中。

Features of lipoprotein metabolism in rabbits, unlike mouse, resemble to human, we planned to make human apoCIII transgenic (Tg) rabbits to reveal its functions on lipoprotein metabolism and atherogenesis. For the first year, we injected more than 500 embryos but failed to obtain any positiveTg rabbits due to low number of living pups born. After that, we modified the protocol of microinjection. In the second year, we successfully got 48 alive pups. Although we still did not get any positive rabbits that carry human apoCIII gene, it is quite possible that we will be successful soon. The work is under way for the further experiment.