Genome analysis of bacteriophage toward botulinum neurotoxin-transfer mechanism

噬菌体C -st是典型的肉毒杆菌C型神经毒素转化噬菌体。虽然噬菌体c-st基因组已经测序，但其生物学和基因组方面的信息还不多。为了扩大我们对噬菌体水平基因转移的理解，我们研究了噬菌体c-st的核心基因组。首先对噬菌体结构蛋白进行分析，结构蛋白的基因组模块为噬菌体c-st基因组的98.5-157.5 kbp。第二，将噬菌体c-st的基因组与相关的噬菌体c-468的基因组进行比较，噬菌体c-st的43-57 kbp和116.5-123 kbp基因组与噬菌体468基因组非常相似。第三，噬菌体基因组分配必需基因位于噬菌体c-st基因组的175.5 ~ 178.5 kbp。根据这些结果，我推测核心基因组为噬菌体c-st基因组的1-43 kbp、57-116.5 kbp和123-186 kbp区域。

Phage c-st is typical Clostridium botulinumtype C neurotoxin-converting phage. Although the phage c-st genome has been sequenced, no much information is available in its biology and genome. To extend our understanding of horizontal gene transfer by this phage, we investigated in the core genome of phage c-st. First, analyzing the phage structural proteins, the genome module for the structural proteins was 98.5-157.5 kbp of phage c-st genome. Second, comparing the genome of phage c-st with that of a related phage c-468, the 43-57 kbp and 116.5-123 kbp of phage c-st genome were very similar to phage 468 genome. Third, the essential genes for phage genome partitioning were revealed to be located 175.5-178.5 kbp of phage c-st genome. According to these results, I speculated that the core genome was the 1-43 kbp, 57-116.5 kbp, and 123-186 kbp regions of phage c-st genome.