Analysis of the mechanisms of allopurinol-induced severe cutaneous adverse reactions

为了研究别嘌醇或苯妥英钠是否会产生药物-蛋白质加合物，将这两种药物分别与不同的人肝组织孵育。结果表明，细胞色素P450活性和细胞内谷胱甘肽的浓度对苯妥英钠药物-蛋白质加合物的形成具有重要意义。此外，尽管P450活性参与别嘌醇代谢的研究尚未见报道，但本研究结果提示P450活性在药物-蛋白质加合物的生成中起作用。

To investigate whether allopurinol or phenytoin generate drug-protein adduct, each drugs were incubated with various human liver tissues. As a result, it is suggested that cytochrome P450 activities and the concentration of glutathione in cells are of importance for generation of drug-protein adduct formation with phenytoin. In addition, although it have not been reported that P450 activities are involved in the allopurinol metabolism, the result of this study suggested that P450 activities played a role in generation of the drug-protein adduct.