Basic research for development of cancer prevention by inhibitory effect of food constituents on the signal transduction pathways associated with carcinogenesis

MEK-ERK或PI 3 K-Akt信号转导通路在许多癌症中被加速，并且被认为是非常重要的分子靶点，不仅用于癌症治疗，而且用于癌症预防。为开发针对食品成分致癌相关信号转导途径的抗癌新策略，研究了油菜素和脱氢姜酮对人结肠癌细胞的生长抑制作用，并分析了其分子机制。从十字花科蔬菜如卷心菜和花椰菜中提取的拟南芥素通过抑制PI 3 K-Akt通路抑制人结肠癌HT-29细胞的生长。脱氢姜酮，来源于生姜，被称为姜黄素的结构半类似物，已知其抑制MEK-ERK通路。脱氢姜酮抑制HT-29细胞的生长与细胞内活性氧的积累有关。此外，PI 3 K抑制剂与组蛋白脱乙酰酶抑制剂的组合显示可用于人子宫内膜癌HEC-1A细胞的生长抑制。

MEK-ERK or PI3K-Akt signal transduction pathways are accelerated in many cancers and considered to be the very important molecular targets not only for the cancer therapy but also for the cancer prevention. To develop a novel strategy for cancer prevention targeting the major signal transduction pathways associated with carcinogenesis by food constituents, growth inhibitory effect of brassinin ordehydrozingerone on human colon cancer cells was studied and the molecular mechanisms were analyzed. Brassinin, derived from cruciferous vegetables like cabbage or broccoli, repressed the growth of human colon cancer HT -29 cells mediated by inhibition of PI3K-Akt pathway . Dehydrozingerone, derived from a ginger, and is known as a structural half analogue of curcumin, which is known to inhibit MEK-ERK pathway. Dehydrozingerone repressed the growth of HT -29 cells in association with accumulation of intracellular reactive oxygen species (ROS). In addition, combination of a PI3K inhibitor with histone deacetylase inhibitor was shown to be useful for growth inhibition of human endometrial carcinoma HEC-1A cells.