Development of simultaneous micro analysis method for muscle relaxants in biological samples by UPLC-MS/MS

本研究建立了超高效液相色谱串联质谱（UPLC-MS/MS）同时分析肌肉松弛剂的快速、灵敏方法。我们的仪器能够在正、负电离模式下同时选择目标化合物的反应监测，从而实现了UPLC-MS/MS对中枢作用肌肉松弛剂的同时分析。另一方面，采用ESI（电喷雾电离）模式的LC-MS/MS难以定量外周非去极化肌肉松弛剂。然而，本研究建立的采用ESI-APCI（大气压化学电离）混合电离模式的UPLC-MS/MS同时分析全血中非去极化肌肉松弛剂的方法具有更好的定量效果。

In this study, rapid and sensitive methods for simultaneous analysis of muscle relaxants by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) were developed.Simultaneous analysis of central-acting muscle relaxants by UPLC-MS/MS has been realized by the capability of our instrument for simultaneous selected reaction monitoring of the target compounds in both positive and negative ionization modes. On the other hand, quantification of peripheral non-depolarizing muscle relaxants was difficult by LC-MS/MS with ESI (Electrospray ionization) mode. However, the developed simultaneous analysis method of non-depolarizing muscle relaxants in whole blood by UPLC-MS/MS with ESI-APCI (Atmospheric pressure chemical ionization) mix ionization mode in this study showed better quantitativity.