Basic study of autologous transplantation of ex vivo expanding macrophages to secondary lymphedema

我们试图建立术后淋巴水肿小鼠模型，但未能成功。可能的原因是：(1)用蓝色染料很难看到淋巴管。(2)行走时未见明显水肿。(3)淋巴结检测困难。接下来，我们使用了糖尿病伤口模型，这是已经建立的淋巴功能受损模型之一。我们关注的是他汀类药物，它可能是巨噬细胞的刺激剂。他汀类药物通过促进巨噬细胞功能显著改善糖尿病创面模型淋巴管生成。

We tried to establish postoperative lymphedema mouse model but it was failed within the period. The possible reasons are (1)difficulty of visualizing lymphatics by blue dye. (2)edema was not significantly observeded because of quaruped walking. (3)difficulty of the detection of lymph nodes. We next used diabetic wound mo del which is one of the established model of impaired lymphatic function. We focused on statin which can be possible stimulator of macrophages. Statin application significantly improve lymphangiogenesis in diabetic wound model via promoting macrophage func tion.