Detection and functional analysis of cardiac stem cells

在这项研究中，我们进一步分析了包括RAE28/Phc1在内的多梳-rgoup(PcG)复合体1的分子作用，并试图鉴定心脏干细胞，重点是Ginin的表达水平。PCG复合体1通过泛素-蛋白酶体系统调节蛋白质的稳定性，并通过染色质调节转录。我们在这里发现了一个新的PcG复合体，它与心脏中的Pcgf5和心脏中的高细胞亚群双生。由于双生高细胞位于心脏心房，在此之前发现了心脏干细胞的存在，这些细胞被推定为心脏干细胞的候选者。另一方面，双生素在S期细胞中的表达很高，这表明双生子高表达细胞可能是成人心脏中的增殖细胞。

In this study we further analyzed a molecular role for the Polycomb-rgoup (PcG) complex 1 including Rae28/Phc1 and attempted to identify cardiac stem cells focusing on the expression level of Geminin. PcG complex 1 regulates protein stability through the ubiquitin-proteasome system as well as transcription through the chromatin regulation. We here identified a novel PcG complex with Pcgf5 in the heart and Gemininhighcellular subpopulations in the heart. Since Gemininhighcells reside in the cardiac atrium, in which there previously shown was the presence of cardiac stem cells, these are presumed to be candidates for cardiac stem cells. On the other hand, expression of Geminin is high in S-phase cells, suggesting an intriguing possibility that Gemininhigh cells are proliferating cells in the adult heart.