Analysis of non-coding RNA expression in stem and progenitor cells in mouse testes

在小鼠中，我们发现异染色质形成的显著进展发生在成年雄性生殖细胞中从干细胞向祖细胞的转变。此外，基因表达模式也发生了显着变化的过渡。鉴于非编码RNA在异染色质形成中起重要作用，导致基因表达谱的改变，我们分析比较了成年男性生殖细胞中干细胞和祖细胞中miRNA和长链非编码RNA的表达谱。我们发现了几种在每个细胞群中特异性表达的miRNA和长非编码RNA。

In mice, we found that the significant progression of heterochromatin formation occurred at the transition from stem to progenitor cells in adult male germ cells. In addition, the gene expression pattern was also significantly changed at the transition. Since it has been reported that non-coding RNA plays an important role in heterochromatin formation, resulting in changing of gene expression profiles, we analyzed and compared the expression profiles of miRNA and long non-coding RNA between stem cells and progenitor cells in adult male germ cells. We found several miRNA and long non-coding RNA that were specifically expressed in each of the cell populations.