Study of a Rapid Test for Mycobacterium tuberculosis Complex Using an Immunochromatographic Assay

研究了一种简便、快速的M.结核菌复合体的MPB64的免疫层析检测。已经报道了几种分泌的分枝杆菌蛋白。在这些分泌蛋白中，MPB 64已被鉴定为M.结核复合体特异性分泌蛋白。该试剂盒是新研制的快速鉴别伊卡.结核复合体和除M.结核病（MOTT）。初步研究了M.结核复合体与基于液体培养基的培养系统的组合。在这种方法中，我们可以很容易地检测到M。结核病综合征其次，我们研究了从临床标本中直接检测的方法。在浓缩处理和净化的沉淀物后，将样品置于试剂盒的测试孔中。直接检测法能正确检测出M.结核复合体和MOTT，标本中含有大量的M。结核病综合征该系统可用于M.肺结核综合征

We investigated simple and rapid test for M. tuberculosis complex by using immunochromatographic assay (ICA) to detect MPB64. Several secreted mycobacterial proteins have been reported. Among these secreted proteins, MPB64, has been identified as a M. tuberculosis complex-specific secretory protein. This kit is the newly developed ICA for rapid discrimination between the M. tuberculosis complex and the mycobacteria other than M. tuberculosis (MOTT). Primary investigation, we study rapid identification of the M. tuberculosis complex in combination with the culture systems based on liquid media. In this method, we can easily detect the M. tuberculosis complex from MOTT. Secondary, we investigated direct testing method from clinical specimens. Specimens were placed into the test well of kits, after concentrating the sediments processed and decontaminated. Direct testing method could correctly detect between the M. tuberculosis complex and MOTT, specimens contain high volume of M. tuberculosiscomplex. With a few issue, this system is useful for the rapid isolation and detection of M. tuberculosis complex.