Lipoprotein lipidomics of oxystress-induced diseases by using supercritical fluid technologies

我们成功地开发了一种综合脂蛋白脂质谱分析方法。构建的高分辨率、高通量系统采用超临界流体色谱/质谱（SFC/MS）和在线超临界流体萃取系统。为了确定该系统对真实样本的适用性，我们对心肌梗死易感兔和高危心肌梗死患者进行了脂质组学分析。获得了疾病特异性概况，并鉴定了标记代谢物。此外，使用我们开发的方法，我们确定了代谢变化，不能通过使用传统的方法来识别，目标是通过对脂蛋白组分进行分析血清脂质。

We successfully developed an analysis method for comprehensive lipoprotein lipid profiling. The constructed high-resolution and high-throughput system uses supercritical fluid chromatography/mass spectrometry (SFC/MS) and on-line supercritical fluid extraction system. To determine the applicability of this system to real samples, we performed lipidomics analyses on myocardial infarction-prone rabbits and high-risk myocardial infarction patients. Disease-specific profiles were obtained and marker metabolites were identified. Furthermore, using our developed method, we identified metabolic variations that could not be identified by using the conventional method, which targets serum lipids by performing analyses on lipoprotein fractions.