Oxidized lipid metabomics by online-supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry

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A supercritical fluid (SCF) possesses features such as low viscosity and high diffusivity. Therefore, it has suitable properties for use as a mobile phase in chromatography and a media in extraction. Supercritical fluid chromatography (SFC) would be possible to perform rapid and high-resolution separation of complicate metabolites which is difficult to separate by GC and HPLC. Supercritical fluid extraction (SFE) is mild and high-efficient, and it is suitable for unstable metabolites by solvent extraction. Therefore, to apply them to biometrics analysis, it would be necessary to establish analytical system using unique character of SCF. In this study, we have developed the technology toward application of online SFE-SFC/MS system to oxidized lipid profiling and applied the analysis system to lipidomics in various diseases.