Development of microfluidic mixer for time-resolved spectroscopy applicable to low-yield biomolecules

为了了解生物分子反应的分子机制，必须通过实时测量来揭示分子沿着反应的动力学。然而，蛋白质的产率通常很低，为ug-mg，并且由于相对较大的消耗，时间分辨测量难以适用于许多生物分子反应。在本研究中，开发了适用于低产量蛋白质样品的时间分辨光谱，并应用于观察折叠、膜运输和酶促反应。

To understand the molecular mechanism of biomolecular reactions, the dynamics of the molecules along the reaction have to be revealed by the real-time measurements. However, the yield of protein is usually very low, ug-mg, and the time-resolved measurements is hard to apply for many biomolecular reactions because of the relatively large consumption. In this research, the time-resolved spectroscopies applicable to the low-yield protein samples were developed and applied to observe the folding, membrane transport and enzymatic reactions.