Contribution of transcriptional corepressor ETO2 to the epigenome formation in erythroblasts

在K562人红细胞系中过量表达ETO 2以及染色质免疫沉淀分析显示ETO 2直接抑制代表性红细胞基因，包括珠蛋白基因。随后，我们建立了从脐血CD 34阳性细胞向红系分化的系统。通过使用该系统，我们证明了短发夹RNA（shRNA）介导的脐带血CD 34阳性细胞来源的初级成红细胞中ETO 2的敲低导致珠蛋白基因的显著上调。

Overexpression of ETO2 as well as chromatin immunoprecipitation analysis in K562 human erythroid cell line revealed that ETO2 directly represses representative erythroid genes, including globin genes. Subsequently, we established erythroid differentiation system from cord blood CD34-positive cells. By using the system, we demonstrated that short hairpin RNA (shRNA)-mediated knockdown of ETO2 in cord blood CD34-positive cell-derived primary erythroblasts resulted in the significantupregulation of globin genes.