Fabrication of Complex Hydrogel Materials with Microscale Patterns for Cell Organization

我们开发了微流控系统，用于制备嵌入具有不同物理化学性质的微米图案的复杂水凝胶材料。制备了复杂的水凝胶纤维、条纹片、细胞尺寸的ECM颗粒和平面图案。例如，这些材料被应用于原代肝细胞和非实质细胞的共培养，形成含有ECM颗粒的异质球体，以及通过组合这些单元材料来制备相对较大的组织。此外，还提出了使用由水凝胶制成的微流控装置来制备血管组织模型的策略。

We developed microfluidic systems for preparing complex hydrogel materials embedding micrometer-patterns with different physicochemical properties. Complex hydrogel fibers, stripe-patterned sheets, cell-sized ECM particles, and planar patterns were fabricated. These materials were applied to, for example, the coculture of primary hepatocytes and non-parenchymal cells, the formation of heterogeneous spheroids incorporating ECM particles, and the preparation of relatively large tissues by combining these unit materials. In addition, strategies for preparing vascular tissue models have been proposed using microfluidic devices made of hydrogel.