Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction

在本研究中，我们旨在通过制备新型刺激响应型聚阳离子释放表面来提高反转染法的效率，这是一种经济、低毒的基因导入方法。以半胱氨酸为刺激物检测多聚阳离子释放。结果，通过使用对细胞无毒的含半胱氨酸的水溶液，聚阳离子从表面释放。此外，293T细胞在表面有很好的粘附性，表面显示出一定的细胞毒活性。这种功能表面可能成为基因导入的实验平台。

In this study, we aimed to improve the efficiency of reverse transfection, one of the economic and low cytotoxic gene introduction methods, by preparing novel stimuli-responsive polycation releasing surface. Polycation release was examined by using cysteine as stimuli. As a result, polycation was released from the surface by applying cysteine containing aqueous solution which was nontoxic to cells. Moreover, 293T cells were well adhered to the surface and surface showed any cytotoxic activities. This functional surface would be potentially useful as experimental platform for gene introduction.