Isolation for an antibacterial substance produced by Vibrio vulnificus and its characterization

分离得到了对创伤弧菌有抑制作用的抗菌物质。在本实验室保存的51株家畜培养物中，创伤弧菌L-9对45株创伤弧菌具有抑菌活性。创伤弧菌L-9产生的抗菌物质对几种香料弧菌、革兰氏阴性菌如大肠杆菌、革兰氏阳性菌如金黄色葡萄球菌、枯草芽孢杆菌、罗伊氏乳杆菌和几种香料乳球菌均有抑制作用。抗菌物质对蛋白酶K敏感，在pH 3 ~ 6范围内稳定，在高盐浓度（24%）条件下稳定。经超滤测定，抗菌物质的表观分子质量小于2000 Da。上述结果表明，创伤弧菌L-9产生的抗菌物质是一种具有广谱抗菌活性的新型细菌素。

We isolated for antibacterial substance with an inhibitory activity against Vibrio vulnificus. V. vulnificus L-9 showed antibacterial activity against 45 V. vulnificus strains among 51 stock cultures maintained by our laboratory. The antibacterial substance produced by V. vulnificus L-9 inhibited several strains of Vibrio spices, gram negative bacteria such as Escherichia coli, gram positive bacteria such as Staphylococcus aureus，Bacillus subtilis, Lactobacillus reuteri and several strains of Lactococcus spices. The antibacterial substance was sensitive to Protease K, was stable in the pH 3-6, and wasin the high salt concentration (24%). The apparent molecular mass of antibacterial substance was estimated to be lower than 2,000 Da, by the ultrafiltration. These results indicated that the antibacterial substance produced by V. vulnificus L-9 was a novel bacteriocin with broad spectrum antibacterial activity.