Restoration of extinct plant population using the seed of herbarium specimens and development of specimen management method

对117种555份濒危植物标本的种子进行了胚酶活性测定和发芽试验。结果表明，73种植物中有235种（42%）的种子存活，存活率为62%。现存最古老的种子标本是美丽风毛菊（菲施. ex Hornem.）菲施。87年前收集的。我还检查了标本制备方法对种子活力的影响。在低于40 ℃的温度下干燥处理的标本种子表现出较高的存活率。以及采集标本的野生种群。实生苗群体的遗传多样性显著低于野生群体，但确认了野生群体中丢失的等位基因。

I investigated the enzyme activity of the embryo and germination test of herbarium specimens's seed of endangered plant of 555 specimens of 117 species. As a result, the seeds of 235 specimens (42%) of 73 species (62%) were alive. The oldest survival specimen seed was Saussurea pulchella (Fisch. ex Hornem.) Fisch. that has been collected 87 years ago.I alos examined the effect of specimen preparation method has on the viability of the seed. Specimen seeds subjected to drying treatment at a temperature lower than 40 degrres C showed a high survival rate.And I compared the genetic diversity of seedling populations of specimens of Vincetoxicum pycnostelma Kitag. and the wild populations from which the specimens were collected. Genetic diversity of the seedlings were significantly lower than that of wild populations, but the alleles which were lost from the wild population were confirmed.