The development of a new treatment for renal cancer based on the induction of anoikis by protein C inhibitor

本研究利用肝细胞生长因子(HGF)表达人蛋白C抑制物(PC I)的B16黑色素瘤细胞和从肝脏分泌人PC I的转基因小鼠，研究了肝细胞生长因子(HGF)对肝细胞蛋白C抑制物(PC I)表达的影响，以及细胞表达的PC I和宿主血浆PC I对肿瘤生长和转移的影响。我们发现，肝细胞生长因子降低了肝脏中PCI的表达。此外，我们认为细胞表达的PCI和宿主血浆的PCI都抑制肿瘤的生长，并且抑制其转移，而宿主血浆的PCI促进肿瘤转移是通过促进凝血而不是抑制失巢凋亡来实现的。

We examined the effect of hepatocyte growth factor(HGF) on protein C inhibitor(PCI) expression in liver cells, and the effect of cell-expressing PCI and host plasma PCI on the growth and metastasis of cancer using PCI-expressing B16 melanoma cells and PCI gene transgenic mice which secrete human PCI from their liver. We showed that HGF reduces PCI expression in the liver. Further, we suggested that both cell-expressing PCI and host plasma PCI inhibit cancer growth, and cell-expressing PCI inhibits its metastasis, whereas host plasma PCI promotes cancer metastasis by enhancing blood coagulation, not inhibition of anoikis.