Development of novel treatment on masticatory muscle pain with TMD

连续7天向MM注射IL-1 β后，TG中FG标记的P2 X3 R阳性神经元的平均百分比显著增加。连续给予MM IL-1 β受体拮抗剂可减弱TG中P2 X3-IR细胞的增加。MM在300 g压力刺激后的ATP释放也在CMM后显著增强。MM中给予选择性P2 X3，2/3受体拮抗剂A-317491可减轻CMM大鼠HWT的减少。在注射IL-1 β的大鼠中，A-317491（60 µg）注射后30 min也观察到HWT显著增加。这些结果表明，P2 X3 R表达与MM中IL-1 β表达和ATP释放的增强相关，在肌肉过度收缩后MM机械性痛觉过敏中可能具有重要作用。

The mean percentage of FG-labeled P2X3R-positive neurons was significantly increased in TG following successive IL-1beta injections into the MM for 7 days. Successive administration of an IL-1beta receptor-antagonist into the MM attenuated the increase of P2X3-IR cells in the TG. ATP release from MM after 300-g pressure stimulation of MM was also significantly enhanced after CMM. Administration into MM of the selective P2X3,2/3 receptor antagonist A-317491 attenuated the decrement of HWT in CMM rats. A significant increase in HWT was also observed at 30 min after A-317491 (60 µg) injection in IL-1beta-injected rats. These findings suggest that P2X3R expression associated with enhanced IL-1beta expression and ATP release in MM has a possible important role in MM mechanical hyperalgesia after excessive muscular contraction.